The Mouse Albumin ELISA Kit is an in vitro immunoassay for the quantification of albumin circulating in serum or in other appropriately qualified samples from tissue fluids (e.g., saliva, mucosa), or in cultures of mouse cells.
Test Principle:The Mouse Albumin ELISA kit is based on the binding of mouse albumin in samples to two antibodies, one immobilized on the microtiter wells, and the other conjugated to horseradish peroxidase (HRP) enzyme. After a washing step, chromogenic substrate is added and color is developed by the enzymatic reaction of HRP on the TMB substrate, which is directly proportional to the amount of albumin present in the sample. Stopping Solution is added to terminate the reaction, and absorbance at 450nm is then measured using an ELISA microtiter well reader. The concentration of albumin in samples and control is calculated from a curve of standards containing known concentrations of albumin.
Kit Components:A1274-90T1: Microtiter Plate: 1x96 wellsA1274-90T2: Control (98-182ng/ml), 1x650ulA1274-90T3: Standard (20ng/ml), 1x650ulA1274-90T4: Standard (50ng/ml), 1x650ulA1274-90T5: Standard (100ng/ml), 1x650ulA1274-90T6: Standard (200ng/ml), 1x650ulA1274-90T7: Standard (400ng/ml), 1x650ulA1274-90T8: HRP Conjugate, 100X, 1x150ulA1274-90T9: Sample Diluent 20X, 1x10mlA1274-90T10: Wash Solution 100X, 1x10mlA1274-90T11: TMB Substrate, 1x12mlA1274-90T12: Stop Solution, 1x12ml
Storage and Stability:Store all components at 4°C. Stable for 6 months after receipr. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
