The name of “Limulus” is derived from a scientific name of the horseshoe crab; Limulus polyphemus. Four species of horseshoe crabs exist in limited regions of the world. The hemolymph amebocyte is the circulating blood cell of the horseshoe crab. Levin and Bang found out that endotoxin (LPS) induces coagulation of the lysate of hemolymph caused by an anti-LPS factor. Limulus anti-LPS factor (LALF) is a naturally occurring horseshoe crab derived protein that is both bactericidal for gram-negative bacteria and capable of neutralizing LPS. The cyclic LALF-peptide, amino acids 31-52, binds lipid A and neutralizes LPS in vivo and in vitro.
Applications:Suitable for use in biological assays in vivo and in vitro. Dilutions have to be made according to the amount of protein to be inactivated. Further dilutions should be made by using protein stabilized PBS, pH 7 (never use fresh serum/plasma). Be aware: Endotoxin will bind LALF-peptide.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at 4°C and is stable for 12 months at 4°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Stock solution should be stored in aliquots at -70°C in polypropylene tubes. LALF-peptide stock solution should never be thawed more than once. Repeated freeze and thaw cycles will cause loss of LALF-peptide activity.
仕様
Size：100ug
Grade：Purified
Purity：Purified
Form：Supplied as a lyophilized powder in PBS. Reconstitute with sterile, endotoxin free PBS.
