Proprotein Convertase 2 (PCSK2) is a subtilisin-like serine peptidase that processes proteins into biologically active products in the secretory pathway. Like other furin/kexin family members, PCSK2 cleaves its substrates primarily after sites of paired basicaaresidues. Autoactivation of PCSK2 occurs in a post-Golgi compartment of the secretory system. Interaction with the secretory protein 7B2 is required for proper activation of PCSK2. The N-terminal domain of 7B2 stabilizes active PCSK2, and a C-terminal fragment of 7B2 can inhibit PCSK2 activity. rhPCSK2 was coexpressed with 7B2 to facilitate activation of the enzyme. PCSK2 is a major proteolytic processing enzyme of the regulated secretory pathway of the neuroendocrine system, where it generates a number of hormones and neuropeptides. Products of PCSK2 processing include enkephalins, insulin, somatostatin, dynorphin, and LHRH.
Chinese Hamster Ovary cell line, Gly110-Asn638 with a C-terminal 6-His tag. Swiss/UniProt Accession: P16519
SDS-PAGE:69kD, reducing conditions
Activity:Measured by its ability to cleave the fluorogenic peptide substrate pERTKR-AMC.
Specific Activity:~60 pmoles/min/ug
Storage and Stability:Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
