Lysyl oxidase-like protein 3 (LOXL3) is a secreted copper amine oxidase. The N-terminal region of LOXL3 contains four scavenger receptor cysteine- rich (SRCR) domains. The C-terminal region consists of a catalytic domain similar to other lysyl oxidases and a cytokine receptor-like domain. The catalytic domain contains conserved residues required for copper binding and formation of a lysyl tyrosylquinone cofactor. LOXL3 is expressed primarily in epidermis, kidney, aorta, and chondrocytes. The enzyme is expressed at low levels in most other tissues. Like lysyl oxidase, LOXL3 oxidizes the lysine epsilon-amino groups of a number of collagen substrates, and is also active against other primary amines. However, LOXL3 does not require proteolytic processing to be activated.
Source:Chinese Hamster Ovary cell line, CHO-derived Met1-Ile753, with a C-terminal 10-His tag.
SDS-PAGE:90-100kD, reducing conditions
Activity:Measured by its ability to produce to produce hydrogen peroxide during the oxidation of benzylamine.
Specific Activity:≥1.5pmoles/min/ug
Endotoxin Level: ≤1EU/1ug (LAL)
Storage and Stability:May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：10ug
Source Antigen：Recombinant, Chinese Hamster Ovary cell line
Grade：Purified
Purity：~90% (SDS-PAGE, reducing conditions)
Form：Supplied as a liquid in MES, sodium chloride.
