Frizzled-8 is one of at least ten seven- transmembrane (7TM) glycoproteins of the Frizzled family of Wnt receptors. Frizzled proteins are thought to be G-protein-coupled. Wnt engagement, with low density lipoprotein receptor- related proteins LRP-5 or LRP-6 acting as co-receptors, stabilizes b-catenin and promotes gene transcription that is important in development and tissue maintenance. Human Frizzled-8 cDNA encodes 694aa including a 27aa signal peptide, a 247aa extracellular domain (ECD), the 7TM region (aa276-605), and a C-terminal cytoplasmic domain with a PDZ binding motif (aa606-694). The ECD includes a cysteine-rich region (CRD, aa30-151) that binds Wnts and is highly conserved among Frizzleds, and a linker region. Within aa28-172, human Frizzled-8 shares 99%, 99%, 90% and 85% aa identity with mouse, rat, Xenopus and zebrafish Frizzled-8, respectively. It also shares 82% aa identity with human Frizzled-5. Frizzleds can form homodimers or selective hetero-oligomers with other family members, which can involve the TM regions and possibly the CRD. During mouse development, Frizzled-8 is expressed in tissues that are important for organizing the anterior-posterior axis. Interactions of Frizzled-8 with several non-Wnt ligands have been identified. Interaction with Frizzled-8 and LRP-6 allows R-spondins to activate b-catenin signaling pathways, while interaction with IGFBP-4 (insulin-like growth factor binding protein or CTGF (connective tissue growth factor) inhibits Wnt signaling. These ligands bind the extracellular CRD of Frizzled-8, blocking Wnt binding. The recombinant Frizzled-8 CRD has also been used to block Wnt signaling and inhibit growth of teratocarcinomas.
SDS-PAGE:55-65kD, reducing conditions
Activity:Measured by its binding ability in a functional ELISA. In a 100ul reaction mixture containing biotinylated recombinant mouse Wnt-3a or Wnt-5a at 50-100ng/ml and recombinant human Frizzled 8/Fc Chimera dilutions at 0.1-8000ng/ml, the concentration of recombinant human Frizzled 8/Fc Chimera that produces 50% of the optimal binding response is found to be ~7-28ng/ml.l.
Storage and Stability:Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：50ug
Source Antigen：Recombinant, Human Frizzled 8 (Ala25-Pro172) at the N-terminus, IEGRMD and human IgG1 (Pro100-Lys330) at the C-terminus derived in Chinese Hamster Ovary cell line.
Grade：Highly Purified
Purity：≥95% as determined by SDS-PAGE and visualized by silver stain. Endotoxin: ≤1EU/1ug (LAL)
Form：Supplied as a lyophilized powder from PBS, pH 7.2. Reconstitute with 100ul sterile PBS.
