Bone morphogenetic protein 10 (BMP-10), along with BMP-9, GDF-5, -6, and -7, belongs to a subgroup of TGF-b superfamily proteins that signal through heterodimeric complexes composed of type I and type II BMP receptors. Proteolytic removal of the propeptide from the 60kD proprotein yields a 12kD mature BMP-10 which forms disulfide-linked nonglycosylated homodimers. In transfectants, BMP-10 can also be secreted as a proprotein which binds to matrix fibrillin. Mature mouse BMP-10 shares 98% and 100ªsequence identity with human and rat BMP-10, respectively, and 48%-64% with mouse BMP-9, GDF-5, -6, and -7. BMP-10 is critical for the proper development of the heart and first appears at the onset of trabeculation and chamber formation. Homozygous BMP-10 knockout mice die in utero due to arrested cardiac development. BMP-10 is required for maintaining expression of the cardiogenic transcription factors NKX2.5 and MEF2C in developing myocardium and promoting the Notch-dependent growth of embryonic cardiomyocytes. NKX2.5 itself negatively regulates BMP-10 expression in cardiac myocytes. BMP-10 in the postnatal heart promotes increased cardiomyocyte and heart size and is upregulated in hypertrophied ventricles. Mature BMP-10 accumulates within cardiomyocytes in association with the sarcomere protein Tcap. BMP-10 induces signaling through ALK-1, BMPR-IA, BMPR-IB, and BMPR-II in transfectants and non-cardiac cell lines. Deletion of BMPR-IA or BMP-10 causes similar cardiac morphogenetic abnormalities. In dermal endothelial cells, BMP-10 induces migration, proliferation, and gene expression typically associated with ALK-1.
Source:Mouse Bmp10 (Asn314-Arg421; Accession #Q9R229) expressed in Chinese hamster ovary (CHO) cells.
SDS-PAGE:10kD, reducing conditions
Activity:Measured by its ability to induce alkaline phosphatase production by MC3T3-E1 mouse osteoblastic cells. The ED50 for this effect is typically 15-75ng/ml.
Endotoxin Level:≤1EU/1ug (LAL)
Storage and Stability:Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with 4mM HCl containing at least 0.1% HSA or BSA. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：25ug
Source Antigen：Recombinant, Chinese Hamster Ovary cell line
Grade：Highly Purified
Purity：>95% by SDS-PAGE under reducing conditions and visualized by silver stain.
Form：Supplied as a lyophilized powder from HCl, BSA. Reconstitute with 4mM HCl, 0.1% human or bovine serum albumin.
