PHPT1 is a 125aa enzyme belonging to the Janus protein family. Existing as a monomer in the cytoplasm, PHPT1 is an EDTA-insensitive phosphohistidine phosphatase. Overexpression of PHPT1 leads to specific phosphohistidine phosphatase activity towards phosphopeptide I, with no activity detected towards phosphotyrosine, phosphothreonine and phosphoserine peptides.
Source:Recombinant corresponding to aa1-125 of human PHPT1 protein, fused to His-tag at N-terminus, expressed in E.coli.
Molecular Weight: 15.9kD (145aa) confirmed by MALDI-TOF
Storage and Stability:May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size:100ug
Source Antigen:Recombinant, E.coli
Grade:Purified
Purity:~95% (SDS-PAGE).
Form:Supplied as a liquid in 20mM Tris-HCl, pH 8.0, 0.2M sodium chloride, 2mM DTT, 10% glycerol.