NCK adaptor protein 1, also known as NCK, belongs to the adaptor family of proteins. The protein encoded by this gene is one of the signaling and transforming proteins containing Src homology 2 and 3 (SH2 and SH3) domains. It is located in the cytoplasm and is an adaptor protein involved in transducing signals from receptor tyrosine kinases to downstream signal recipients such as RAS. Overexpression of Nck resulted in transformation of NIH 3T3 and 3Y1 rat fibroblast.
Source:Recombinant corresponding to aa1-377 of human NCK1 6x his tagged expressed in E.coli.
Molecular Weight: 45kD (397aa), confirmed by MALDI-TOF
Storage and Stability:May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing.. Store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：100ug
Source Antigen：Recombinant, E.coli
Grade：Purified
Purity：~90% (SDS-PAGE).
Form：Supplied as a liquid in 20mM Tris-HCl, pH 8.0, 1mM DTT, 20% glycerol, 1mM EDTA, 50mM sodium chloride.
