ITPA, also known as Inosine triphosphate pyrophosphatase, is an enzyme that catalyzes the pyrophosphohydrolysis of both ITP (inosine triphosphate) and dITP (deoxyinosine triphosphate) to IMP (inosine monophosphate) and diphosphate. IMP can be used as a substrate for purine nucleotide pathways. IMP can be phosphorylated to ITP, and ITPA can regulate the concentration of ITP in the cell by converting ITP back to IMP. Defects in ITPA result in ITPA deficiency which is thought to be inherited and is characterized by an over-accumulation of ITP in erythocytes, leukocytes and fibroblasts.
Source:Recombinant corresponding to aa10194 of human ITPA protein, fused to His-tag at N-terminus, expressed in E.coli.
Molecular Weight: 23.7kD (215aa) confirmed by MALDI-TOF
Enzyme Activity:Not determined. This product is recommended for use in applications that do not require a catalytically active form of the protein.
Storage and Stability:May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：100ug
Source Antigen：Recombinant, E.coli
Grade：Highly Purified
Purity：~95% (SDS-PAGE).
Form：Supplied as a liquid in 20mM Tris-HCl, pH 8.0, 10% glycerol.
