CRK is a member of signaling adapter protein family that binds to several tyrosine-phosphorylated proteins. This protein is involved in many cellular processes including apoptosis, proliferation, and differentiation. It has a modular domain architecture consisting of an SH2 followed by two SH3 domains (src-homology domains). The N-terminal SH2 domain of this protein functions as a positive regulator of transformation whereas the C-terminal SH3 domain functions as a negative regulator of transformation.
Source:Recombinant corresponding to aa1-204 of human CRK protein, fused to His-tag at N-terminus, expressed in E.coli.
Molecular Weight: 25kD (224aa) confirmed by MALDI-TOF
Storage and Stability:May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：10ug
Source Antigen：Recombinant, E.coli
Grade：Highly Purified
Purity：~95% (SDS-PAGE).
Form：Supplied as a liquid in 20mM Tris-HCl, pH 8.0, 10% glycerol.
