Heparan sulfate is a sulfated glycosaminoglycan with the repeating disaccharide units of -4HexA1,4GlcNAcb1-. It is usually attached to the protein cores of proteoglycans found on the cell membrane and in the extracellular matrix where it binds to a variety of protein ligands and regulates a wide range of biological activities. Heparan sulfate has a domain structure where sulfated regions are interspaced with less or non-sulfated regions. Heparin shares the backbone structure with heparan sulfate but contains no non-sulfated regions. Heparinases are a family of lyases that release unsaturated oligosaccharides from heparin and heparan sulfate upon digestion. Heparinase I recognizes highly sulfated regions and is more specific for heparin. Heparinase II digests both heparin and heparan sulfate. Heparinase III prefers less-sulfated regions and is more active on heparan sulfate.
Source:E. coli-expressed recombinant protein corresponding to Gln25-Pro659 of P. heparinus Heparinase III, with an N-terminal Met and 6-His tag
Activity: Measured by its ability to liberate oligosaccharides from heparin.
Specific Activity: >3,000 pmoles/min/ug
Storage and Stability:Aliquot to avoid repeated freezing and thawing. Store at -70°C. Aliquots are stable for 6 months at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：10ug
Source Antigen：Recombinant, E.Coli
Grade：Highly Purified
Purity：≥ 95% (SDS-PAGE, reducing conditions; 5ug protein loaded per lane; visualized by colloidal Coomassie blue staining). Endotoxin: <1.0 EU/1ug (LAL).
Form：Supplied as a sterile-filtered liquid in Tris-buffered saline containing glycerol
