Full-length recombinant protein of human XIAP with N-terminal FLAG tag expressed in a Baculovirus infected Sf9 cell expression system (NM_001167).
Specific Activity:0.0015pmol/min/ug.
Assay Conditions:4mM ATP, 5mM MgCl2 (both required for reaction), 1mM DTT, E1 (0.5ug), E2 (2.8ug), Biotinylated Ubiquitin (1ug) in 50mM Tris pH 7.4.
Applications:Suitable for use in conjunction with E1 and E2 for the study of enzyme kinetics, screeninginhibitors and selectivity profiling. Other applications not tested.
Recommended Dilutions:Optimal dilutions to be determined by the researcher.
Storage and Stability:Aliquot to avoid repeated freezing and thawing. Store at -70°C. Aliquots are stable for 6 months after receipt at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size:20ug
Source Antigen:Recombinant, Sf9 cell
Grade:Purified
Purity:~65%
Form:Supplied as a liquid in 40mM Tris-HCl, pH 8.0, 110mM sodium chloride, 2.2mM KCl, 80ng/ul FLAG peptide, 3mM DTT, 20% glycerol.