Source:Recombinant corresponding to aa150-end of human SMURF 1, fused with N-terminal FLAG tag expressed in a Baculovirus infected Sf9 cell expression system (NM_020429).
Specific Activity:0.0055pmol/min/ug Assay conditions: 50ul reaction mix (4mM ATP, 5mM MgCl2, 1mM DTT, E1 (0.5ug), E2 (2.8ug), Biotin-Ubiquitin (0.5ug)and Smurf1 (0-1ug) in 50mM Tris pH 7.4)incubate for 30min at 37ºC, then add to streptavidin coated plates and incubate for30min at 37ºC. Add antibody against Smurf1, incubate 1 hour. Finally, incubate 1 hour with the Europeum-labeled secondary antibody. Time resolved fluorescence measured at room temperature (lambda-exc=330 nm, lambda-em=620 nm).
Applications:Suitable for use in conjunction with E1 and E2 for the study of enzyme kinetics, screeninginhibitors and selectivity profiling. Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Storage and Stability:Aliquot to avoid repeated freezing and thawing. Store at -70°C. Aliquots are stable for at least 6 months at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：20ug
Source Antigen：Recombinant, Sf9 cell
Grade：Purified
Purity：Purified ≥80%
Form：Supplied as a liquid in 25mM Tris-HCl, pH 8.0, 100mM sodium chloride, 0.05% Tween 20, 100ug/ml FLAG peptide, 3mM DTT, 20% glycerol.
