85-1556-76　PKM2, N-terminal HIS-Tag, Recombinant, Mouse (Pyruvate Kinase, Isozymes M1/M2, Pyruvate Kinase Muscle Isozyme, Cytosolic Thyroid Hormone-binding Protein, CTHBP, Opa-interacting Protein 3, OIP-3, Pyruvate Kinase 2/3, Thyroid Hormone-binding Protein 1, THBP1, Tumor M2-PK, p58, PKM, OIP3, PK2, PK3) 20ug　P9545-33

特徴

• Source:Recombinant corresponding to aa2-end of mouse rPKM2 with N-terminal His tag expressed in E. coli (NM_011099).
• Specific Activity:11pmol/min/ug. A 50ul PKM2 reaction is conducted in a buffer containing 50mM Tris, pH 7.4, 100mM magnesium chloride, 250mM potassium chloride, 40mM ADP, 100mM phosphoenolpyruvate (PEP) at RT for 15 minutes. ATP production is detected using Kinase-Glo Luminescent Kinase Assay Platform.
• Applications:Suitable for use in the study of enzyme kinetics, screening inhibitors and selectivity profiling. Other applications not tested.
• Recommended Dilution:Optimal dilutions to be determined by the researcher.
• Storage and Stability:Aliquot to avoid repeated freezing and thawing. Store at -70°C. Aliquots are stable for 6 months at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

仕様

• Size：20ug
• Source Antigen：Recombinant, E. coli
• Grade：Purified
• Purity：Purified ≥90%
• Form：Supplied as a liquid in 40mM Tris-HCl, pH 8.0, 110mM sodium chloride, 2.2mM potassium chloride, 200mM imidazole, 3mM DTT, 20% glycerol.
• Molecular Weight Non Ab：58
• Swiss Prot Number：P52480
• EU Commodity Code：30021019
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