Source:Recombinant corresponding to aa883-end of human PTPRS with N-terminal GST tag expressed in E. coli (BC104812).
Specific Activity:223.2pmol/min/mg. Assay buffer: 50mM HEPES, pH 7.4, 2mM EDTA, 3mM DTT, 100mM sodium chloride, 50mM pNPP. The specific activity of PTPRS was determined using pNPP. Enzyme reaction condition: 20mM pNPP, 2min incubation at 30°C, 1ug enzyme.
Applications:Suitable for use the study of enzyme kinetics, screening inhibitors and selectivity profiling. Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Storage and Stability:Aliquot to avoid repeated freezing and thawing. Store at -70°C. Aliquots are stable for at least 6 months at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：20ug
Source Antigen：Recombinant, E. coli
Grade：Purified
Purity：Purified ≥70%
Form：Supplied as a liquid in 25mM Tris-HCl, pH 8.0, 100mM sodium chloride, 0.05% tween-20, 3mM DTT, 20% glycerol.
