Source:Recombinant corresponding to full length human PKG1 beta with N-terminal His tag expressed in Baculovirus infected Sf9 cell expression system (NM_006258).
Specific Activity:0.85pmoles/min/ug. Enzyme reaction is conducted in a buffer containing 50mM HEPES, pH 7.5, 10mM magnesium chloride, 1mM EGTA, 200uM ATP, 0.01% Brij-35, 2uM substrate (SER/THR 14) at RT for 1hour.
Applications:Suitable for use in the study of enzyme kinetics, screening inhibitors and selectivity profiling. Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Storage and Stability:Aliquot to avoid repeated freezing and thawing. Store at -70°C. Aliquots are stable for at least 12 months at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：10ug
Source Antigen：Recombinant, Sf9 cell
Grade：Purified
Purity：Purified ~70%
Form：Supplied as a liquid in 25mM Tris-HCl, pH 7.5, 100mM sodium chloride, 0.05% tween 20, 3mM DTT, 20% glycerol.
