Source:Recombinant corresponding to aa530-end of human MUSK, fused with N-terminal His-Tag, expressed in Baculovirus infected Sf9 cell expression system (NM_005592).
Specific activity:6.9pmole/min/ug. Assay conditions: Add 50ul reaction mix (MUSK diluted in 1x kinase buffer supplemented with 0.2mM ATP) to wells coated with substrate. Incubate for 1 hour. Add Europeum-labeled anti p-Tyr antibody and incubate for 1 hour. Time resolved fluorescencemeasured at RT at lambda-exc=330±40nm and lambda-em=620±10nm.
Applications:Suitable for use in the study of enzyme kinetics, screening inhibitors, and selectivity profiling. Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Storage and Stability:Aliquot to avoid repeated freezing and thawing. Store at -70°C. Aliquots are stable for at least 12 months at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：10ug
Source Antigen：Recombinant, Sf9 cell
Grade：Purified
Purity：Purified ≥60%
Form：Supplied as a liquid in 25mM Tris-HCl, pH 7.5, 100mM sodium chloride, 0.05% tween 20, 3mM DTT, 20% glycerol.
