Source:Recombinant corresponding to aa2-end of human MKK6 with N-terminal GST tag expressed in a Baculovirus infected Sf9 cell expression system (NM_002758.3).
Specific Activity:Enzyme reaction is conducted in a buffer containing 50mM HEPES, pH7.5, 10mM magnesium chloride, 1mM EGTA, 200uM ATP, 0.01% Brij-35, 100ng of inactive MAPK14, 2uM substrate (SER/THR 4) at RT for 1 hour. Developer solution was added to reaction and reaction was stopped after 1 hour of incubation at RT.
Applications:Suitable for use in the study of enzyme kinetics, screening inhibitors and selectivity profiling. Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Storage and Stability:Aliquot to avoid repeated freezing and thawing. Store at -70°C. Aliquots are stable for at least 6 months at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：20ug
Source Antigen：Recombinant, Sf9 cell
Grade：Purified
Purity：Purified ≥80%
Form：Supplied as a liquid in 25mM Tris-HCl, pH 8.0, 100mM sodium chloride, 0.05% tween-20, 3mM DTT, 20% glycerol.
