Source:Recombinant corresponding to aa781-end of human Jak3 with N-terminal GTS tag expressed in Baculovirus expression sstem (NM_000215).
Specific Activity:118pmol/min/ug. Assay conditions: 50mM HEPES, pH7.5, 10mM MgCl2, 1mM EGTA, 200uM ATP, 0.01% Brij-35, 2uM substrate at RT for 1hour. Developer solution was added to reaction and reaction was stopped after 1 hour of incubation at RT.
Applications:Suitable for use in the study of enzyme kinetics, screening inhibitors, and selectivity profiling. Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Storage and Stability:Aliquot to avoid repeated freezing and thawing. Store at -70°C. Aliquots are stable for at least 6 months at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：10ug
Source Antigen：Recombinant, baculovirus
Grade：Purified
Purity：Purified ≥60%
Form：Supplied as a liquid in 25mM Tris-HCl, pH8.0, 100mM NaCl, 0.05% tween-20, 1mM DTT, 20% glycerol.
