Source:Recombinant corresponding to CD33 signal peptide(aa1-16) and human cathepsin S (aa17-331) with C-terminal HIS tag expressed in FreeStyle 293-F cells (NM_004079.3).
Specific Activity:8100pmol/min/ng. Assay Condition: 50mM sodium acetate, pH 4.5, 0.25M sodium chloride, 5mM DTT, 10uM substrate (Mca-R-P-K-P-V-E-Nval-W-R-K (Dnp)-NH2), 10 minutes at RT. Enzyme was preactivated in 50mM sodium acetate, pH 4.5, 0.25M sodium chloride, 5mM DTT buffer for 2 hours at RT prior to reaction.
Applications:Suitable for use in the study of enzyme kinetics, screening inhibitors and selectivity profiling. Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Storage and Stability:Aliquot to avoid repeated freezing and thawing. Store at -70°C. Aliquots are stable for at least 6 months at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：10ug
Source Antigen：Recombinant, FreeStyle 293-F cell
Grade：Purified
Purity：Purified ≥90%
Form：Supplied as a liquid in 25mM Tris-HCl, pH 8.0, 100mM sodium chloride, 0.05% tween-20, 10% glycerol.
