Human cathepsin F (NM_003793), Cathepsin F (aa20-end), withC-terminal His-tag, MW = 53kD (more than 58kD after post-translational modifications), expressed in FreeStyle 293-F cells.
Specific Activity:~0.1pmole/min/ug.
Assay Condition:Enzyme activity was measured in a buffer containing 100mM sodium acetate, pH 5.5, 2mM EDTA, 8mM DTT, 0.05% Brij, and 20uM substrate (Z-FRAMC), with 3.75-30ng/ul Cathepsin F. Reaction was done at 30°C for 40-120 min, and fluorescence signal was measured at Ex360nm/Em460nm. Enzyme was preactivated in 50mM sodium acetate, pH 4.5, 5mM DTT buffer for 90 min at RT prior to reaction.
Applications:Suitable for use in the study of enzyme kinetics, screening inhibitors and selectivity profiling. Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Storage and Stability:Aliquot to avoid repeated freezing and thawing. Store at -70°C. Aliquots are stable for at least 6 months at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：50ug
Source Antigen：Recombinant, Freestyle 293-F cell
Grade：Purified
Purity：≥90% by SDS-PAGE
Form：Supplied as a liquid in 45mM sodium acetate, 135mM sodium chloride,10% glycerol.
