Source:Recombinant corresponding to full length human BRSK2 with N-terminal His tag expressed in baculovirus expression system (NM_003957).
Specific Activity:412pmoles/min/ug. Enzyme reaction is conducted in a buffer containing 50mM HEPES, pH 7.5, 10mM MgCl2, 1mM EGTA, 200uM ATP, 0.01% Brij-35 and 2uM substrate (SER/THR 21) at RT for 1 hour.
Applications:Suitable for use in the study of enzyme kinetics, screening inhibitors, and selectivity profiling. Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Storage and Stability:Aliquot to avoid repeated freezing and thawing. Store at -70°C. Aliquots are stable for at least 6 months at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：10ug
Source Antigen：Recombinant, baculovirus
Grade：Purified
Purity：Purified ≥70%
Form：Supplied as a liquid in 25mM Tris-HCl, pH 8.0, 100mM sodium chloride, 0.05% tween-20, 10% glycerol.
