ATP citrate lyase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. The enzyme is a tetramer (relative molecular weight approximately 440,000) of apparently identical subunits. It catalyzes the formation of acetyl-CoA and oxaloacetate from citrate and CoA with a concomitant hydrolysis of ATP to ADP and phosphate. The product, acetyl-CoA, serves several important biosynthetic pathways, including lipogenesis and cholesterogenesis. In nervous tissue, ATP citrate-lyase may be involved in the biosynthesis of acetylcholine.
Human ATP citrate lyase (ACLY) full length, with N-terminal GST tag, expressed in baculovirus expression system. Accession: NM_001096.
Full length enzyme corresponding to aa2-110 of human ATP citrate lyase (ACLY) with N-terminal GST tag, expressed in baculovirus expression system. Accession: NM_001096.
Specific Activity:≥300pmole/min/ug
Assay Condition:Performed with ACLY titrated from 120ng/rxn to 0ng/rxn in 2-fold dilutions.
Applications:Suitable for use in enzyme kinetics, inhibitor screening and selectivity profiling. Other applications not tested.
Recommended Dilutions:Optimal dilutions to be determined by the researcher.
Storage and Stability:Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：10ug
Source Antigen：Recombinant, baculovirus
Grade：Purified
Purity：~90% by SDS-PAGE
Form：Supplied as a liquid in 40mM Tris-HCl, pH 8.0, 110mM sodium chloride, 2.2mM potassium chloride, 3mM DTT, 20% glycerol.
