Recombinant corresponding to full length human ACC2 with C-terminal His tag expressed in Baculovirus infected Sf9 cell (NM_001093).
Specific Activity:~20 pmoles/min/ug. A 25ul ACC2 reaction is conducted in a buffer containing 30mM HEPES, pH 7.4, 2mM MgCl2, 2mM sodium citrate, 1mM DTT, 100uM ATP, 12mM NaHCO3 and 20uM acetyl-Co-A at 30ºC for 45 minutes. ATP reduction is detected using Kinase-Glo Luminescent Kinase Assay Platform.
Applications:Suitable for use inthe study of enzyme kinetics, screening inhibitors and selectivity profiling. Other applications not tested.
Recommended Dilutions:Optimal dilutions to be determined by the researcher.
Storage and Stability:Aliquot to avoid repeated freezing and thawing. Store at -70°C. Aliquots are stable for 6 months after receipt at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：10ug
Source Antigen：Recombinant, Sf9 cells
Grade：Purified
Purity：~30%
Form：Supplied as a liquid in 45mM Tris-HCl, pH 8.0, 500mM sodium chloride, 2.2mM KCl, 3mM DTT, variable amounts of Imidazole, 10% glycerol.
