Carcinoembryonic Antigen (CEA) is a 180-200kD glycoprotein expressed at high level in the epithelial cells of the embryonic colon. The expression is stopped after birth. CEA serum content is low in normal population and only slightly elevated in heavy smokers. Increased serum CEA level was found associated with colorectal carcinoma, gastric carcinoma, pancreatic carcinoma, lung carcinoma, and breast carcinoma (3, 4, 5, 6). CEA has been used as a biomarker for carcinoma. However elevated CEA level not necessarily indicates neoplastic conditions, since high CEA expression has been observed in non-neoplastic conditions such as ulcerative colitis, pancreatitis and cirrhosis. Clinically, CEA level is mainly used to monitor the tumor recurrence after surgical resection of carcinoma (1, 2, 7).
Kit Components:1. CEA Microtiter Plate, 1x96 wells 2. Ab (HRP), 1x12ml3. CEA Standard – 160ng, 1x1 vial 4. CEA Standard – 80ng, 1x1 vial5. CEA Standard – 40ng, 1x1 vial6. CEA Standard – 20ng, 1x1 vial7. CEA Standard – 10ng, 1x1 vial8. CEA Standard – 5ng, 1x1 vial9. CEA Standard – 2.5ng, 1x1 vial10. CEA Standard – 0ng, 1x1 vial11. Substrate A, 1x10ml12. Substrate B, 1x10ml13. Stop Solution, 1x15ml14. Wash Buffer (20X), 1x60ml
Storage and Stability:Store other components at 4°C. Stable for 6 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
