IL-4 is produced by T cells, NK T cells, gamma delta cells, and mast cells (1). Target cells include B cells, T cells, and macrophages (1). IL-4 induces the polarization of naïve helper T cells into the TH2 phenotype (1,2). IL-4 also promotes B cell proliferation, antibody class switching and the production other TH2 cytokines including IL-5 and IL-9. IL-4 induced TH2 polarization is important in developing humoral immunity against extracellular pathogens (1) and is involved in the development of allergy and asthma (3). IL-4 binds to two distinct receptors, the Type I receptor and Type II receptor. Type I receptor is a heterodimer consisting of IL-4Rα chain and the common gamma chain, gc (4,5). Type II receptor, which is shared with IL-13, is a heterodimer of IL-4Ra and IL-13Ra1. Signaling initiated via Type I receptor results in the activation of Jak1/Stat6, Jak3 and the PI3K/Akt pathways (4). The Type II receptor activates the Jak1/Stat6 and the Tyk2/Stat3 pathways (4).
Source:Recombinant corresponding to His21-Ser140 from mouse IL-4 expressed in human 293 cells (NP_067258).
Molecular Weight:Recombinant mIL-4 contains no "tags" and the nonglycosylated protein has a calculated MW of 13,557. DTT-reduced and non-reduced protein migrate as 16kD polypeptide due to glycosylation. The expected amino-terminal HIHGC of recombinant mIL-4 was verified by amino acid sequencing.
Biological Activity:Determined in an HT-2 cell proliferation assay. The ED50 is 100-400pg/ml.
Storage and Stability:Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile buffer or ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：10ug
Source Antigen：Recombinant, 293 cells
Grade：Highly Purified
Purity：~98% (SDS-PAGE, reduced and non-reduced). Endotoxin: ≤0.01ng/1ug.
Form：Supplied as a lyophilized powder from PBS, pH 7.2. Carrier Free.
