Hepatocyte growth factor (HGF) is a member of the plasminogen related growth factor family. The HGF protein is a dimer formed from an alpha and beta subunit linked by a disulfide bond. Both subunits originate from the endoproteolytic cleavage of a common precursor which is biologically inactive. HGF is expressed in Kupffer cella and sinusoidal endothelial cells in the liver and pancreas.
Source:DNA sequence encoding the complete human Hepatocyte Growth Factor protein isoform 1 (comprising the signal peptide, alpha and beta chains) was expressed in modified human 293 cells.
Biological. Activity:The ED50 of HGF is typically 20-40 ng/ml as measured in a cell proliferation assay using the human HepG2 cell line.
Applications: Suitable for use in Western Blot. Other applications not tested.
Recommended Dilution:Western Blot: Under reducing conditions, the HGF protein migrates as a broad band between ~80 and 110kD in SDS-PAGE due to post-translational modifications, in particular glycosylation. The unmodified HGF has a predicted monomeric molecular mass of 79.7kD. The protein separates into a number of isoforms with a pI between 6 and 9 in 2D PAGE. The unmodified HGF protein has a predicted pI of 8.2. Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O or PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：5ug
Source Antigen：Recombinant, Modified human 293 cells
Grade：Highly Purified
Purity：≥95% (SDS-PAGE, Coomassie Brilliant Blue)
Form：Supplied as lyophilized powder from PBS, 1%HSA, 10% trehalose. Reconstitute with 500ul sterile PBS.
