Thioredoxin 1 (Trx) is a low molecular weight redox protein. Trx contains a redox active disulfide/dithiol group within the conserved Cys-Gly-Pro-Cys active site. It is involved in the first unique step in DNA synthesis. Trx also provides control over a number of transcription factors affecting cell proliferation and death through a mechanism referred to as redox regulation.
Recombinant enzyme corresponding to aa2-109 of Thioredoxin-1 fused to His-tag at N-terminus, was expressed in E. coli.
Specific Acitivity:>70 A650/cm/min/mg, obtained by measuring the increase of insulin precipitation in absorbance at 650nm resulting from the reducution of insulin.
Storage and Stability:May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：50ug
Source Antigen：Recombinant, E. coli
Grade：Highly Purified
Purity：≥95% (SDS-PAGE). Purified by using conventional chromatography techniques.
Form：Supplied as a liquid in 20mM Tris HCl, pH 8.0, 1mM DTT, 10% glycerol.
