DHPS, which belongs to the deoxyhypusine synthase family of proteins, is important for the first step in the hypusine biosynthesis pathway. This protein catalyzes the NAD-dependent transfer of the butylamine moiety of spermidine to the epsilon-amino group of a specific lysine residue of the EIF5A precursor protein to form the intermediate deoxyhypusine residue. Recombinant DHPS protein was expressed in E. coli.
Source:Recombinant corresponding to aa1-369 of human DHPS 6x His tagged expressed in E. coli.
Enzyme Activity:Not determined. This product is recommended for use in applications that do not require a catalytically active form of the protein.
Applications:Suitable for use in Western Blot. Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size:100ug
Source Antigen:Recombinant, E. coli
Grade:Highly Purified
Purity:~ 95% (SDS-PAGE). Purified by using conventional chromatography techniques.
Form:Supplied as a liquid in 20mM Tris HCl, pH 8.0, 20% glycerol, 0.1M NaCl.