CA3, also known as carbonic anhydrase III, is an enzyme that catalyses rapid conversion of carbon dioxide to bicarbonate and protons (CO2 + H2O = HCO3 + H+). This protein is involved in a variety of biological processes, including respiration, calcifica-tion, acid-base balance, bone resorption and the formation of aqueous humor, cerebrospinal fluid, saliva and gastric juice. It contains a zinc ion in their active site and the primary function of this enzyme is known to maintain acid-base balance in blood and other tissues, and to help transport carbon dioxide of tissues. Recombinant CA3 protein was expressed in E. coli.
Source:Recombinant corresponding to aa1-260 of human CA3 expressed in E. coli.
Enzyme Activity:Not determined. This product is recommended for use in applications that do not require a catalytically active form of the protein.
Applications:Suitable for use in Western Blot. Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size:100ug
Source Antigen:Recombinant, E. coli
Grade:Highly Purified
Purity:~ 90% (SDS-PAGE). Purified by using conventional chromatography techniques.
Form:Supplied as a liquid in 20mM Tris HCl, pH 8.0, 1mM DTT, 10% glycerol.