TNF, alpha is an E. coli-derived recombinant protein corresponding to the C-terminal 157aa of human Tumor Necrosis Factor Alpha (TNFa), a cytokine which exerts cytotoxic effects on a wide range of tumor cells.
ED50: ≤ 0.05 ng/ml as determined by an assay measuring cytolysis of L929 cells in the presence of Actinomycin D.
Source:Recombinant corresponding to human TNF, alpha, expressed in E. coli.
Molecular Weight:~17kD (157aa sequence)
Endotoxin: ≤0.1ng/ug
Applications:Suitable for use in ELISA, Functional Assays and Western Blot. Other applications not tested.
Recommended Dilution:ELISA: 0.2-0.4ng/wellWestern Blot: 1.5-3ng/laneFunctional Assays: 0.05-20ng/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile buffer or ddH2O. Aliquot and store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：50ug
Source Antigen：Human, Recombinant, E. coli
Grade：Highly Purified
Purity：Purified: ~98% (SDS PAGE, HPLC).
Form：Supplied as a lyophilized powder from 3mM tris, pH8.0. No preservative added. Reconstitute with 500ul sterile dH2O.
