S-Adenosyl-homocysteine hydrolyzes in acid solution (0.1M HCl, 100°C, over 90 minutes) to form S-ribosylhomocysteine. It is not hydrolyzed in neutral alkaline solutions (stable in 0.1M NaOH at 25°C over 10 minutes), but oxidation is more rapid in alkaline solution. S-Adenosyl-homocysteine is slowly oxidized to the sulfoxide as a solid and in solution. It is protected from oxidation by thiodiglycol; neutral solutions containing thiodiglycol are stable for long periods at 25°C.
EC Number:213-560-8
Molecular Weight:384.4
Applications:Suitable for use in ELISA. Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
