PAI-1 (plasminogen activator inhibitor-1) is a serine protease inhibitor and member of the serpin family, which acts as the primary inhibitor of tissue plasminogen activator (tPA) and urokinase plasminogen activator (uPA), and hence fibrinolysis.
Increased levels of PAI-1 are associated with diabetes, cardiovascular disease and an increased occurrence of thrombosis in obesity, and can be induced by obesity related factors including insulin, glucose and VLDL (very-low-density lipoprotein).
Source:Recombinant corresponding to human PAI-1, expressed in E. coli.
Molecular Weight:~42.7kD (379aa sequence)
Activity:Determined by its inhibitory effect against single chain tPA induced cleavage of a chromogenic substrate in Imidazole Buffer at 37oC. Half maximal inhibition against 1.0ug/ml of single chain tPA was obtained at a concentration of 2.0ug/ml.
Endotoxin: ≤1EU/ug
Applications:Suitable for use in Functional Assays. Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile buffer or ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：10ug
Source Antigen：Recombinant, E. coli
Grade：Highly Purified
Purity：~98% (SDS PAGE, HPLC).
Form：Supplied as a lyophilized powder. Preservative free. Reconstitute with 100ul sterile dH2O.
