Human macrophage/monocyte chemotactic and activating factor (MCAF) (also known as macrophage chemoattractant protein-1 (MCP-1), plays an important role in the inflammatory response of monocytes and macrophages
Recombinant corresponding to human MCP-1, expressed in E. coli. Swiss/UniProt Accession: P13500
Molecular Weight:~8.6kD (76aa sequence)
Applications:Suitable for use in Functional Assays, ELISA, and Western Blot. Other applications not tested.
Recommended Dilutions:Functional Assays: 10-100ng/mlELISA: 0.2-0.4ng/wellWestern Blot: 1.5-3.0ng/laneOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized and reconstituted products are stable for 6 months after receipt at -20°C. Reconstitute with sterile dH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Form:Supplied as a lyophilized powder. Preservative free. Reconstitute with 200ul sterile dH2O. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. It is recommend that the vial is gently mixed after reconstitution