Interleukin-6 (IL-6) is a multifunctional pro-inflammatory cytokine, produced by a wide variety of cells including B lymphocytes, T lymphocytes, monocytes/macrophages, fibroblasts and endothelial cells and plays a major role in the regulation of immune responses, haematopoiesis and the induction of acute phase responses.
Recombinant protein corresponding to chicken IL-6, fused to His-Tag, expressed in E. coli.
Applications:Suitable for use in ELISA, Functional Assays and In vitro Assays. Other applications not tested.
Recommended Dilution:Functional Assays: Induces corticosterone secretion in vivo. In vitro Assays: Induces proliferation of the IL-6 dependant murine hybridoma cell line 7TD1 Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：50ug
Source Antigen：Recombinant, E. coli.
Grade：Purified
Purity：~85% by Ni Chelate chromatography
Form： Supplied as a liquid in PBS. No preservative added.
