Interleukin-1 receptor antagonist (IL-1RA) is a naturally occurring protein which inhibits the activity of IL-1 alpha and IL-1 beta by competitively blocking binding to cell surface receptors for IL-1.
Recombinant protein corresponding to human IL-1RA, expressed in E. coli.
Applications:Suitable for use in Functional Assays, ELISA and Western Blot. Other applications not tested.
Recommended Dilution:Functional Assays: 20-40ng/mlELISA: 0.2-0.4ng/well, may be used as the standard for ELISA applications with either a purified human IL-1RA antibody or a biotinylated human IL-1RA antibody
Western Blot: 1.5-3.0ng/lane, may be used as a positive control for Western Blotting applications with either a purified human IL-1RA antibody or a biotinylated human IL-1RA antibody
Optimal dilutions to be determined by the researcher.
Biological Activity:In an assay of dose dependent inhibition of IL-1 stimulation of D10S cells, the ED50 was expected to be 20-40ng/ml.
Storage and Stability:Lyophilized and reconstituted products may be stored at -20°C. Stable for 6 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：20ug
Source Antigen：Recombinant, E. coli
Grade：Highly Purified
Purity：≥98% (SDS PAGE). Endotoxin: ≤0.1ng/ug
Form：Supplied as a lyophilized powder from TBS. No preservative added. Reconstitute with 200ul sterile dH2O.
