S-phase kinase-associated protein 1, also known as SKP1, is an F-box protein which functions as a substrate recognition component of the SCF ubiquitin ligase complex. It binds to proteins containing an F-box motif, such as cyclin F, S-phase kinase-associated protein 2, and other regulatory proteins involved in ubiquitin dependent proteolysis. It is also involved in the control of beta-catenin levels and the activity of beta-catenin dependent TCF transcription factors.
Recombinant human SKP1 protein was expressed in E.coli and purified by using conventional chromatography techniques.
Applications:Suitable for use in Western Blot. Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Molecular Weight: 18kD (160aa)
Storage and Stability:May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Source Antigen：Recombinant, E. coli
Grade：Affinity Purified
Purity：~90% by SDS-PAGE
Form：Supplied as liquid in 20mM Tris-HCl buffer, pH8.0, 50mM NaCl, 10% glycerol.
