HEXIM1 is induced by hexamethylene-bis-acetamide in vascular smooth muscle cells. This protein has been shown to form a protein-RNA complex composed of 7SK small nuclear RNA and positive transcription elongation factor b (P-TEFb), which is composed of cyclin-dependent kinase 9 (CDK9) and cyclin T1, and to inhibit the kinase activity of CDK9, thereby suppressing RNA polymerase II-dependent transcriptional elongation.
Recombinant protein corresponding to human HEXIM1with a His-Tag, expressed in E. coli and purified by using conventional chromatography techniques.
Applications:Suitable for use in Western Blot. Other applications not tested.
Recommended Dilutions:Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：100ug
Source Antigen：Recombinant, E. coli
Grade：Affinity Purified
Purity：~90% by SDS-PAGE.
Form：Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, 0.1M sodium chloride, 10% glycerol.
