E. coli Beta-Galactosidase is an inducible tetrameric enzyme coded by the lac Z gene of the lac operon that is often used as a reporter to assess the efficiency of transfection. It is a metalloenzyme that splits lactose into glucose and galactose. It hydrolyzes terminal, non-reducing beta-D-galactose residues in beta-D-galactosides.
Source:Purified protein from E. coli
Molecular Weight:~540kD
Specific Activity:> 600U/mg protein.
Unit Definition:One unit hydrolyses 1umole of p-nitrophenol-b-D-galactoside to p-nitrophenol per minute at 37ºC.
Protein Content:~20-25% of total weight
Applications:Suitable for use in Functional Assays. Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized and reconstituted products may be stored at -20°C. Stable for 6 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：5mg
Source Antigen：Recombinant, E. coli
Grade：Purified
Purity：Purified
Form：Supplied as a lyophilized powder in PBS, 74% sucrose. Reconstitute with 1ml sterile dH2O.
