Visinin-like protein-1, also known as VILIP-1, belongs to a family of neuronal calcium sensor proteins. VILIP-1 is strongly expressed in granule cells of the cerebellum where it associates with membranes in a calcium dependent manner and modulates intracellular signaling pathways of the central nervous system by directly or indirectly regulating the activity of adenylyl cyclase. Also, it is expressed in pancreatic beta-cells. VILIP-1 elevation enhances insulin secretion in cAMP-associated manner. Down-regulation of VILIP-1 decreased cAMP accumulation but increased insulin gene transcription. Recombinant human Visinin-like protein-1 , expressed in E. coli and purified by using conventional chromatography.
Recombinant protein corresponding to aa1-191 of human Visinin-like protein-1 expressed in E. coli (NP_003376)
Applications:Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilutions:Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：100ug
Source Antigen：Recombinant, E.coli
Grade：Affinity Purified
Purity：~95 % by SDS-PAGE Purified by immunoaffinity chromatography. Endotoxin: ≤1.0EU/ug (LAL)
Form：Supplied as liquid in 20mM Tris-HCl buffer, pH8.0, 1mM DTT, 10% glycerol.
