VAMP4, also known as vesicle-associated membrane protein 4, is a member of the synaptobrevin family involved in docking and/or fusion of vesicles with cell membrane. This protein is enriched in the trans-Golgi network and may play a role in trans-Golgi network-to-endosome transport. VAMP4 is involved in the pathway that functions to remove an inhibitor (probably synaptotagmin-4) of calcium-triggered exocytosis during the maturation of secretory granules. Recombinant human VAMP4 protein, fused to His-tag at C-terminus, , expressed in E. coli and purified by using conventional chromatography.
Source:Recombinant human VAMP4, 1aa-115, His-tagged, expressed in E. coli
Applications:Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size:50ug
Source Antigen:Recombinant, E.coli
Grade:Affinity Purified
Purity:~90% by SDS-PAGE Purified by immunoaffinity chromatography.
Form:Supplied as liquid in 20 mM Tris-HCl buffer, pH 8.0, 0.1 M NaCl, and 20% glycerol