PTEN, also known as phosphatase and tensin homolog, is a tumor suppressor that is mutated in a large number of cancers at high frequency. This protein acts as both a dual-specificity protein phosphatase and a lipid phosphatase, removing the phosphate in the D3 position of the inositol ring from phosphatidylinositol 3,4,5-trisphosphate. PTEN negatively regulates intracellular levels of phosphatidylinositol-3,4,5-trisphosphate in cells and functions as a tumor suppressor by negatively regulating AKT/PKB signaling pathway. Recombinant PTEN protein, expressed in E. coli and purified by using conventional chromatography techniques.
Source:Recombinant human PTEN, aa1-403, His-tagged, expressed in E. coli
Enzyme Activity:Not determined. This product is recommended for use in applications that do not require a catalytically active form of the protein.
Applications:Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：20ug
Source Antigen：Recombinant, E.coli
Grade：Affinity Purified
Purity：~85% by SDS-PAGE Purified by immunoaffinity chromatography.
Form：Supplied as liquid in 20 mM Tris-HCl buffer, pH 8.0, 1mM EDTA, 2mM DTT, 100mM NaCl, and 20% glycerol
