Interleukin-6, as known as IL-6, is an immunoregulatory lymphokine produced by T-cells in response to antigenic or mitogenic stimulation. It is also a "myokine," a cytokine produced from muscle, and is elevated in response to muscle contraction. IL-6 acts as both a pro-inflammatory and anti-inflammatory cytokine. Smooth muscle cells in the tunica media of many blood vessels also produce IL-6. It is mediated through its inhibitory effects on TNF-alpha and IL-1, and activation of IL-1ra and IL-10. Recombinant interleukin-6 , expressed in E. coli and purifed by conventional chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.
Source:Recombinant human Interleukin 6, aa30-212 His-tagged, expressed in E. coli
Applications:Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：100ug
Source Antigen：Recombinant, E.coli
Grade：Affinity Purified
Purity：~95% by SDS-PAGE Purified by immunoaffinity chromatography.
Form：Supplied as iquid iIn Phosphate Buffered Saline, pH7.4, 10% glycerol
