GSTM1 is a glutathione S-transferase that belongs to the mu class. This enzyme acts by catalyzing the reaction of glutathione with an acceptor molecule to form an S-substituted glutathione (S=sulfur). The reactions utilizing glutathione contribute the transformation of a wide variety of electrophiles, including reactive products of lipid, protein, carcinogens, therapeutic drugs, environmental toxins, and products of oxidative stress. Recombinant GSTM1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Source:Recombinant corresponding to mouse GSTM1, his-tagged, expressed in E. coli.
Enzyme Activity: Specific activity is > 40,000 pmol/min/ug, and is defined as the amount of enzyme that conjugates 1.0 u mole of 1-chloro-2,4-dinitrobenzene (CDNB) with reduced glutathione per minute at pH 6.5 at 25°C.
Storage and Stability:May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：100ug
Source Antigen：Recombinant, E.coli
Grade：Affinity Purified
Purity：~95% (SDS-PAGE) Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in 20mM Tris-HCl, pH8.0, 1mM DTT 10% glycerol.
