CX3CL1, also known as fractalkine. CX3CL1 has a unique CX3C cysteine motif near the amino terminus and is the first member of a fourth branch of the chemokine superfamily. Unlike other known chemokines, CX3CL1 is a type 1 membrane protein containing a chemokine domain tethered on a long mucin like stalk. It is expressed in a membrane-bound form on activated endothelial cells and mediates attachment and firm adhesion of T cells, monocytes and NK cells. Also, CX3CL1 elicits its adhesive and migratory functions by interacting with the chemokine receptor CX3CR1. Recombinant human CX3CL1, fused to His-tag at N-terminus, , expressed in E. coli and purified by using conventional chromatography techniques.
Source:Recombinant human CX3CL1/Fractalkine, aa25- 100, His-tagged, expressed in E. coli
AA Sequence:MGSSHHHHHH SSGLVPRGSH MQHHGVTKCN ITCSKMTSKI PVALLIHYQQ NQASCGKRAI ILETRQHRLF CADPKEQWVK DAMQHLDRQA
Enzyme Activity:Not determined. This product is recommended for use in applications that do not require a catalytically active form of the protein.
Applications:Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：100ug
Source Antigen：Recombinant, E.coli
Grade：Affinity Purified
Purity：~90% by SDS-PAGE Purified by immunoaffinity chromatography.
Form：Supplied as liquid in Phosphate buffered saline, pH7.4, 10% glycerol
