FEN-1, also known as Flap structure-specific endonuclease 1, removes 5' overhanging flaps in DNA repair and processes the 5' ends of Okazaki fragments in lagging strand DNA synthesis. The protein is a member of the XPG/RAD2 endonuclease family and is one of ten proteins essential for cell-free DNA replication. FEN-1 is highly homologous to yeast Rad2. The C-terminal region of FEN-1 may bind to PCNA, thus allowing FEN-1 to function as an exonuclease in DNA replication. Recombinant human FEN-1 protein was expressed in E. coli and purified by using conventional chromatography techniques.
Recombinant protein corresponding to aa1-380 from human FEN-1 protein expressed in E. coli (NP_004102).
Enzyme Activity:Not determined. This product is recommended for use in applications that do not require a catalytically active form of the protein.
Storage and Stability:May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：100ug
Source Antigen：E. coli
Grade：Affinity Purified
Purity：~90% (SDS-PAGE) Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in 20mM Tris-HCl, pH 8.0, 1mM DTT, 10% glycerol
