Carbonic anhydrase 1(CA1) is a zinc metalloenzyme that catalyses reversible hydration of CO2 (CO2 + H2O ↔ HCO3- + H+). This protein is fundamental to many biological processes such as cellular respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, salvia, and gastric acid. It is most abundant in erythrocytes and is a very early marker for erythroid differentiation. Recombinant human Carbonic anhydrase1, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.
Recombinant protein corresponding to human Carbonic anhydrase1, N-terminal his-tagged, expressed in E. coli (NP_001122301).
Enzyme Activity:Not determined. This product is recommended for use in applications that do not require a catalytically active form of the protein.
Storage and Stability:Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Source Antigen：Recombinant, E. coli
Grade：Affinity Purified
Purity：~95% (SDS-PAGE) Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in 20 mM Tris-HCl, pH 8.0, 1mM DTT, 10% glycerol.
