CCL22/MDC is a small cytokine belonging to the CC chemokine family. This protein displays chemotactic activity for natural killer cells, chronically activated T lymphocytes, monocytes and dendritic cells. It interacts with cell surface chemokine receptors CCR4. This chemokine may play a role in the trafficking of activated T lymphocytes to inflammatory sites and other aspects of activated T lymphocyte physiology. Recombinant human CCL22/MDC, fused to His-tag at N-terminus, was expressed as insoluble protein aggregate in , expressed in E. coli and purified by conventional chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.
Source:Recombinant human CCL22/MDC, aa25-93, His-tagged, expressed in E. coli
AA Sequence:MGSSHHHHHH SSGLVPRGSH MGPYGANMED SVCCRDYVRY RLPLRVVKHF YWTSDSCPRP GVVLLTFRDK EICADPRVPW VKMILNKLSQ
Applications:Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size:100ug
Source Antigen:Recombinant, E.coli
Grade:Affinity Purified
Purity:~95% by SDS-PAGE Purified by immunoaffinity chromatography.
Form:Supplied as liquid in phosphate-buffered Saline, PBS, pH7.4, 10% glycerol