Aurora Kinase B is a member of a family of mitotic serine/threonine kinases. Aurora Kinase B associates with chromosomes during prophase prior to relocalizing to the spindle at anaphase. Aurora Kinase B regulates chromosome segregation through the control of microtubule-kinetochore attachment and cytokinesis. Expression of Aurora Kinase B during the G2/M phase transition is tightly coordinated with histone H3 phosphorylation, while overexpression in many kinds of cancers. Recombinant human Aurora Kinase B, fused to His-tag at N-terminus, , expressed in E. coli and purified by using conventional chromatography techniques.
Source:Recombinant human Aurora Kinase B, aa1- 344, His-tagged, expressed in E. coli
Enzyme Activity:Not determined. This product is recommended for use in applications that do not require a catalytically active form of the protein.
Applications:Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer
仕様
Size:100ug
Source Antigen:Recombinant, E.coli
Grade:Affinity Purified
Purity:~90% by SDS-PAGE Purified by immunoaffinity chromatography.