Human Elastase is a 28.5kD serine protease that has been purified from human neutrophils. Elastase is also present in the pancreas, leukocytes and serum.
Molecular Weight:~28.5kD
Activity:20-22 U/mg protein
Unit Definition:One unit is defined as the amount of enzyme that hydrolyzes one umole/minute of MeO-Suc-Ala-Ala-Pro-Val-pNA at 25ºC, in 100mM Tris-HCL, pH 7.5 with 500mM sodium chloride.
Applications:Suitable for use in ELISA. Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size:100ug
Source Antigen:Human Neutrophils
Grade:Highly Purified
Purity:≥95% (SDS PAGE)
Form:Supplied as a liquid in 50mM sodium acetate, pH 5.5, 150mM sodium chloride. No preservative added.